QIAGEN pHClearII crystallisation screen on a 96 well plate (Pipetted in columns)

A1 (1)

1.0 M Lithium chloride
0.1 M Citric acid
4.0


A2 (9)

1.0 M Lithium chloride
0.1 M MES
10 %(w/v) PEG 6000
6.0

A3 (17)

1.0 M Lithium chloride
0.1 M TRIS
20 %(w/v) PEG 6000
8.0

A4 (25)

0.1 M Citric acid
5 %(v/v) Isopropanol
4.0


A5 (33)

0.1 M MES
10 %(v/v) Isopropanol
6.0


A6 (41)

0.1 M TRIS
20 %(v/v) Isopropanol
8.0


A7 (49)

0.8 M Na/K phosphate
5.0



A8 (57)

1.0 M Na/K phosphate
6.3



A9 (65)

1.4 M Na/K phosphate
7.5



A10 (73)

1.0 M Sodium malonate
4.0



A11 (81)

1.9 M Sodium malonate
5.0



A12 (89)

2.9 M Sodium malonate
6.0



B1 (2)

1.0 M Lithium chloride
0.1 M Citric acid
5.0


B2 (10)

1.0 M Lithium chloride
0.1 M HEPES
10 %(w/v) PEG 6000
7.0

B3 (18)

1.0 M Lithium chloride
0.1 M BICINE
20 %(w/v) PEG 6000
9.0

B4 (26)

0.1 M Citric acid
5 %(v/v) Isopropanol
5.0


B5 (34)

0.1 M HEPES
10 %(v/v) Isopropanol
7.0


B6 (42)

0.1 M BICINE
20 %(v/v) Isopropanol
9.0


B7 (50)

0.8 M Na/K phosphate
5.6



B8 (58)

1.0 M Na/K phosphate
6.9



B9 (66)

1.4 M Na/K phosphate
8.2



B10 (74)

1.5 M Sodium malonate
4.0



B11 (82)

2.4 M Sodium malonate
5.0



B12 (90)

3.4 M Sodium malonate
6.0



C1 (3)

1.0 M Lithium chloride
0.1 M MES
6.0


C2 (11)

1.0 M Lithium chloride
0.1 M TRIS
10 %(w/v) PEG 6000
8.0

C3 (19)

1.0 M Lithium chloride
0.1 M Citric acid
30 %(w/v) PEG 6000
4.0

C4 (27)

0.1 M MES
5 %(v/v) Isopropanol
6.0


C5 (35)

0.1 M TRIS
10 %(v/v) Isopropanol
8.0


C6 (43)

0.1 M Citric acid
30 %(v/v) Isopropanol
4.0


C7 (51)

0.8 M Na/K phosphate
6.3



C8 (59)

1.0 M Na/K phosphate
7.5



C9 (67)

1.8 M Na/K phosphate
5.0



C10 (75)

1.9 M Sodium malonate
4.0



C11 (83)

2.9 M Sodium malonate
5.0



C12 (91)

1.0 M Sodium malonate
7.0



D1 (4)

1.0 M Lithium chloride
0.1 M HEPES
7.0


D2 (12)

1.0 M Lithium chloride
0.1 M BICINE
10 %(w/v) PEG 6000
9.0

D3 (20)

1.0 M Lithium chloride
0.1 M Citric acid
30 %(w/v) PEG 6000
5.0

D4 (28)

0.1 M HEPES
5 %(v/v) Isopropanol
7.0


D5 (36)

0.1 M BICINE
10 %(v/v) Isopropanol
9.0


D6 (44)

0.1 M Citric acid
30 %(v/v) Isopropanol
5.0


D7 (52)

0.8 M Na/K phosphate
6.9



D8 (60)

1.0 M Na/K phosphate
8.2



D9 (68)

1.8 M Na/K phosphate
5.6



D10 (76)

2.4 M Sodium malonate
4.0



D11 (84)

3.4 M Sodium malonate
5.0



D12 (92)

1.5 M Sodium malonate
7.0



E1 (5)

1.0 M Lithium chloride
0.1 M TRIS
8.0


E2 (13)

1.0 M Lithium chloride
0.1 M Citric acid
20 %(w/v) PEG 6000
4.0

E3 (21)

1.0 M Lithium chloride
0.1 M MES
30 %(w/v) PEG 6000
6.0

E4 (29)

0.1 M TRIS
5 %(v/v) Isopropanol
8.0


E5 (37)

0.1 M Citric acid
20 %(v/v) Isopropanol
4.0


E6 (45)

0.1 M MES
30 %(v/v) Isopropanol
6.0


E7 (53)

0.8 M Na/K phosphate
7.5



E8 (61)

1.4 M Na/K phosphate
5.0



E9 (69)

1.8 M Na/K phosphate
6.3



E10 (77)

2.9 M Sodium malonate
4.0



E11 (85)

1.0 M Sodium malonate
6.0



E12 (93)

1.9 M Sodium malonate
7.0



F1 (6)

1.0 M Lithium chloride
0.1 M BICINE
9.0


F2 (14)

1.0 M Lithium chloride
0.1 M Citric acid
20 %(w/v) PEG 6000
5.0

F3 (22)

1.0 M Lithium chloride
0.1 M HEPES
30 %(w/v) PEG 6000
7.0

F4 (30)

0.1 M BICINE
5 %(v/v) Isopropanol
9.0


F5 (38)

0.1 M Citric acid
20 %(v/v) Isopropanol
5.0


F6 (46)

0.1 M HEPES
30 %(v/v) Isopropanol
7.0


F7 (54)

0.8 M Na/K phosphate
8.2



F8 (62)

1.4 M Na/K phosphate
5.6



F9 (70)

1.8 M Na/K phosphate
6.9



F10 (78)

3.4 M Sodium malonate
4.0



F11 (86)

1.5 M Sodium malonate
6.0



F12 (94)

2.4 M Sodium malonate
7.0



G1 (7)

1.0 M Lithium chloride
0.1 M Citric acid
10 %(w/v) PEG 6000
4.0

G2 (15)

1.0 M Lithium chloride
0.1 M MES
20 %(w/v) PEG 6000
6.0

G3 (23)

1.0 M Lithium chloride
0.1 M TRIS
30 %(w/v) PEG 6000
8.0

G4 (31)

0.1 M Citric acid
10 %(v/v) Isopropanol
4.0


G5 (39)

0.1 M MES
20 %(v/v) Isopropanol
6.0


G6 (47)

0.1 M TRIS
30 %(v/v) Isopropanol
8.0


G7 (55)

1.0 M Na/K phosphate
5.0



G8 (63)

1.4 M Na/K phosphate
6.3



G9 (71)

1.8 M Na/K phosphate
7.5



G10 (79)

1.0 M Sodium malonate
5.0



G11 (87)

1.9 M Sodium malonate
6.0



G12 (95)

2.9 M Sodium malonate
7.0



H1 (8)

1.0 M Lithium chloride
0.1 M Citric acid
10 %(w/v) PEG 6000
5.0

H2 (16)

1.0 M Lithium chloride
0.1 M HEPES
20 %(w/v) PEG 6000
7.0

H3 (24)

1.0 M Lithium chloride
0.1 M BICINE
30 %(w/v) PEG 6000
9.0

H4 (32)

0.1 M Citric acid
10 %(v/v) Isopropanol
5.0


H5 (40)

0.1 M HEPES
20 %(v/v) Isopropanol
7.0


H6 (48)

0.1 M BICINE
30 %(v/v) Isopropanol
9.0


H7 (56)

1.0 M Na/K phosphate
5.6



H8 (64)

1.4 M Na/K phosphate
6.9



H9 (72)

1.8 M Na/K phosphate
8.2



H10 (80)

1.5 M Sodium malonate
5.0



H11 (88)

2.4 M Sodium malonate
6.0



H12 (96)

3.4 M Sodium malonate
7.0