QIAGEN ProteinComplex crystallisation screen on a 96 well plate (Pipetted in rows)

A1 (1)

0.1M TRIS
25% PEG 350 MME


A2 (2)

0.1 Calcium acetate
0.1M MES
6

A3 (3)

0.1 Lithium chloride
0.1M HEPES
7.5

A4 (4)

0.1M TRIS
25% PEG 400


A5 (5)

0.1M MES
15% PEG 550 MME


A6 (6)

0.2M Sodium chloride
0.1M Na/Khosphate
6.5

A7 (7)

0.1M di-Ammonium sulfate
0.1M TRIS
7.5

A8 (8)

0.2M di-Ammonium sulfate
0.1M Sodium acetate
5.5

A9 (9)

0.2M Sodium chloride
0.1M MES
6

A10 (10)

0.1M Potassium chloride
0.1M TRIS
8

A11 (11)

0.1M HEPES
25% PEG 2000 MME


A12 (12)

0.2M Sodium acetate
0.1M Sodium citrate
5.5

B1 (13)

0.2M Lithium sulfate
0.1M TRIS
7.5

B2 (14)

0.1M Calcium acetate
0.1M Sodium acetate
4.5

B3 (15)

0.2M Sodium acetate
0.1M Sodium citrate
5.5

B4 (16)

0.2M Sodium chloride
0.1M MES
6.5

B5 (17)

0.1M Magnesium chloride
0.1M HEPES
7.5

B6 (18)

10% 2-propanol
0.1M HEPES
7

B7 (19)

0.2M Ammonium Acetate
0.1M Sodium acetate
4

B8 (20)

0.1M Magnesium chloride
0.1M Sodium citrate
5

B9 (21)

0.1M Sodium cacodylate
15% PEG 4000


B10 (22)

0.15M di-Ammonium sulfate
0.1M MES
6

B11 (23)

0.1M HEPES
15% PEG 4000


B12 (24)

0.1M Magnesium chloride
0.1M HEPES
7

C1 (25)

0.15M di-Ammonium sulfate
0.1M TRIS
8

C2 (26)

0.1M Sodium citrate
20% PEG 4000


C3 (27)

0.2M Ammonium Acetate
0.1M Sodium acetate
5

C4 (28)

0.2M Lithium sulfate
0.1M MES
6

C5 (29)

0.1M TRIS
20% PEG 4000


C6 (30)

0.15M di-Ammonium sulfate
0.1M HEPES
7

C7 (31)

20% 2-propanol
0.1M Sodium citrate
5.6

C8 (32)

0.2M Sodium chloride
0.1M TRIS
8

C9 (33)

0.1M Sodium cacodylate
25% PEG 4000


C10 (34)

0.15M di-Ammonium sulfate
0.1M MES
5.5

C11 (35)

0.1 M Sodium cacodylate
25% PEG 4000


C12 (36)

0.2M Potassium iodide
0.1M MES
6.5

D1 (37)

0.2M Sodium chloride
0.1M HEPES
7.5

D2 (38)

12% 1-propanol
0.1M MES
6.5

D3 (39)

0.1M Potassium chloride
0.1M HEPES
7

D4 (40)

0.2M di-Ammonium sulfate
0.1M TRIS
7.5

D5 (41)

0.1M Magnesium chloride
0.1M MES
6

D6 (42)

0.15 M Sodium chloride
0.1M TRIS
8

D7 (43)

0.1M Sodium citrate
15% PEG 6000


D8 (44)

0.1M Magnesium acetate
0.1M Sodium cacodylate
6.5

D9 (45)

5% MPD
0.1M MES
6.5

D10 (46)

0.1M Potassium chloride
0.1M HEPES
7.5

D11 (47)

100 mM TRIS
15% PEG 6000


D12 (48)

0.1M TRIS
20% PEG 6000


E1 (49)

0.1M Magnesium acetate
0.1M Sodium acetate
4.5

E2 (50)

0.1M Sodium citrate
8% PEG 8000


E3 (51)

0.2M Sodium chloride
0.1M Sodium cacodylate
6

E4 (52)

0.1M HEPES
8% PEG 8000


E5 (53)

0.1M TRIS
8% PEG 8000


E6 (54)

0.1M CaAcet
0.1 M Sodium cacodylate
5.5

E7 (55)

0.1M NaPO4
12% PEG 8000


E8 (56)

0.1M MgAcet
0.1M MOPS 7.5
7.5

E9 (57)

0.2M Sodium chloride
0.1M HEPES
7.5

E10 (58)

0.2M di-Ammonium sulfate
0.1M TRIS 8.5
8.5

E11 (59)

0.1M Sodium citrate
20% PEG 8000


E12 (60)

0.2M di-Ammonium sulfate
0.1M MES
6.5

F1 (61)

0.1M HEPES
20% PEG 8000


F2 (62)

0.2M Lithium chloride
0.1M TRIS 8.0
8

F3 (63)

0.1M Magnesium acetate
0.1M MES
6.5

F4 (64)

0.1M HEPES
18% PEG 12000


F5 (65)

0.1M Sodium chloride
0.1M TRIS
8

F6 (66)

0.1M HEPES
15% PEG 20000


F7 (67)

0.1M MES
0.5M di-Ammonium sulfate


F8 (68)

0.1M Sodium acetate
1M di-Ammonium sulfate


F9 (69)

0.1M MES
1M di-Ammonium sulfate


F10 (70)

0.1M TRIS
1M di-Ammonium sulfate


F11 (71)

0.1M Sodium acetate
1.5M di-Ammonium sulfate


F12 (72)

0.1M HEPES
1.5M di-Ammonium sulfate


G1 (73)

0.1M TRIS
1.5M di-Ammonium sulfate


G2 (74)

0.1M Sodium acetate
2M di-Ammonium sulfate


G3 (75)

0.1M HEPES
2M di-Ammonium sulfate


G4 (76)

0.1M TRIS
2M di-Ammonium sulfate


G5 (77)

1M Potassium chloride
0.1M HEPES
7

G6 (78)

0.1M Sodium acetate
2M Na Formate


G7 (79)

0.1M TRIS
3M Na Formate


G8 (80)




0.8M K/NaPO4 pH 7.5
G9 (81)




1.3M K/NaPO4 pH 7.0
G10 (82)




1.6M K/NaPO4 pH 6.5
G11 (83)

0.1M HEPES
1M Sodium acetate


G12 (84)

0.1M HEPES
1M Sodium citrate


H1 (85)

0.1M Sodium citrate
2M Sodium chloride


H2 (86)

0.1M MES
1M Lithium sulfate


H3 (87)

0.1M TRIS
1.6M Lithium sulfate


H4 (88)




1.4M Na malonate pH 6.0
H5 (89)

0.1M TRIS
1.2M Na/K tartrate


H6 (90)

0.1M MES
1.6 M MgSO4


H7 (91)

2% PEG 4000
0.1M Sodium acetate
5

H8 (92)

50mM Calcium acetate
0.1M Sodium cacodylate
6

H9 (93)

0.1M Imidazole
50% MPD


H10 (94)

50mM Magnesium chloride
5% PEG 4000
10% 2-propanol

H11 (95)

0.2M Ammonium Acetate
0.1M HEPES
7.5

H12 (96)

0.1M Sodium chloride
5% MPD
15% ethanol