QIAGEN ProteinComplex crystallisation screen on a 96 well plate (Pipetted in columns)

A1 (1)

0.1M TRIS
25% PEG 350 MME
8


A2 (9)

0.2M Sodium chloride
0.1M MES
20% PEG 2000 MME
6

A3 (17)

0.1M Magnesium chloride
0.1M HEPES
10% PEG 4000
7.5

A4 (25)

0.15M di-Ammonium sulfate
0.1M TRIS
15% PEG 4000
8

A5 (33)

0.1M Sodium cacodylate
25% PEG 4000
5.5


A6 (41)

0.1M Magnesium chloride
0.1M MES
8% PEG 6000
6

A7 (49)

0.1M Magnesium acetate
0.1M Sodium acetate
8% PEG 8000
4.5

A8 (57)

0.2M Sodium chloride
0.1M HEPES
12% PEG 8000
7.5

A9 (65)

0.1M Sodium chloride
0.1M TRIS
8% PEG 20000
8

A10 (73)

0.1M TRIS
1.5M di-Ammonium sulfate
8


A11 (81)





1.3M K/NaPO4 pH 7.0
A12 (89)

0.1M TRIS
1.2M Na/K tartrate
8


B1 (2)

0.1 Calcium acetate
0.1M MES
15% PEG 400
6

B2 (10)

0.1M Potassium chloride
0.1M TRIS
15% PEG 2000 MME
8

B3 (18)

10% 2-propanol
0.1M HEPES
10% PEG 4000
7

B4 (26)

0.1M Sodium citrate
20% PEG 4000
4.5


B5 (34)

0.15M di-Ammonium sulfate
0.1M MES
25% PEG 4000
5.5

B6 (42)

0.15 M Sodium chloride
0.1M TRIS
8% PEG 6000
8

B7 (50)

0.1M Sodium citrate
8% PEG 8000
5


B8 (58)

0.2M di-Ammonium sulfate
0.1M TRIS 8.5
12% PEG 8000
8.5

B9 (66)

0.1M HEPES
15% PEG 20000
7


B10 (74)

0.1M Sodium acetate
2M di-Ammonium sulfate
5


B11 (82)





1.6M K/NaPO4 pH 6.5
B12 (90)

0.1M MES
1.6 M MgSO4
6.5


C1 (3)

0.1 Lithium chloride
0.1M HEPES
20% PEG 400
7.5

C2 (11)

0.1M HEPES
25% PEG 2000 MME
7.5


C3 (19)

0.2M Ammonium Acetate
0.1M Sodium acetate
15% PEG 4000
4

C4 (27)

0.2M Ammonium Acetate
0.1M Sodium acetate
20% PEG 4000
5

C5 (35)

0.1 M Sodium cacodylate
25% PEG 4000
6.5


C6 (43)

0.1M Sodium citrate
15% PEG 6000
5.5


C7 (51)

0.2M Sodium chloride
0.1M Sodium cacodylate
8% PEG 8000
6

C8 (59)

0.1M Sodium citrate
20% PEG 8000
5


C9 (67)

0.1M MES
0.5M di-Ammonium sulfate
6.5


C10 (75)

0.1M HEPES
2M di-Ammonium sulfate
7


C11 (83)

0.1M HEPES
1M Sodium acetate
7.5


C12 (91)

2% PEG 4000
0.1M Sodium acetate
15%MPD
5

D1 (4)

0.1M TRIS
25% PEG 400
8


D2 (12)

0.2M Sodium acetate
0.1M Sodium citrate
5% PEG 4000
5.5

D3 (20)

0.1M Magnesium chloride
0.1M Sodium citrate
15% PEG 4000
5

D4 (28)

0.2M Lithium sulfate
0.1M MES
20% PEG 4000
6

D5 (36)

0.2M Potassium iodide
0.1M MES
25% PEG 4000
6.5

D6 (44)

0.1M Magnesium acetate
0.1M Sodium cacodylate
15% PEG 6000
6.5

D7 (52)

0.1M HEPES
8% PEG 8000
7


D8 (60)

0.2M di-Ammonium sulfate
0.1M MES
20% PEG 8000
6.5

D9 (68)

0.1M Sodium acetate
1M di-Ammonium sulfate
5


D10 (76)

0.1M TRIS
2M di-Ammonium sulfate
8


D11 (84)

0.1M HEPES
1M Sodium citrate
7


D12 (92)

50mM Calcium acetate
0.1M Sodium cacodylate
25% MPD
6

E1 (5)

0.1M MES
15% PEG 550 MME
6.5


E2 (13)

0.2M Lithium sulfate
0.1M TRIS
5% PEG 4000
7.5

E3 (21)

0.1M Sodium cacodylate
15% PEG 4000
6


E4 (29)

0.1M TRIS
20% PEG 4000
8


E5 (37)

0.2M Sodium chloride
0.1M HEPES
25% PEG 4000
7.5

E6 (45)

5% MPD
0.1M MES
15% PEG 6000
6.5

E7 (53)

0.1M TRIS
8% PEG 8000
8


E8 (61)

0.1M HEPES
20% PEG 8000
7


E9 (69)

0.1M MES
1M di-Ammonium sulfate
6.5


E10 (77)

1M Potassium chloride
0.1M HEPES
1M di-Ammonium sulfate
7

E11 (85)

0.1M Sodium citrate
2M Sodium chloride
6


E12 (93)

0.1M Imidazole
50% MPD
7


F1 (6)

0.2M Sodium chloride
0.1M Na/Khosphate
25% PEG 1000
6.5

F2 (14)

0.1M Calcium acetate
0.1M Sodium acetate
10% PEG 4000
4.5

F3 (22)

0.15M di-Ammonium sulfate
0.1M MES
15% PEG 4000
6

F4 (30)

0.15M di-Ammonium sulfate
0.1M HEPES
20% PEG 4000
7

F5 (38)

12% 1-propanol
0.1M MES
10% PEG 5000 MME
6.5

F6 (46)

0.1M Potassium chloride
0.1M HEPES
15% PEG 6000
7.5

F7 (54)

0.1M CaAcet
0.1 M Sodium cacodylate
12% PEG 8000
5.5

F8 (62)

0.2M Lithium chloride
0.1M TRIS 8.0
20% PEG 8000
8

F9 (70)

0.1M TRIS
1M di-Ammonium sulfate
8


F10 (78)

0.1M Sodium acetate
2M Na Formate
5


F11 (86)

0.1M MES
1M Lithium sulfate
6.5


F12 (94)

50mM Magnesium chloride
5% PEG 4000
0.1M MES
10% 2-propanol

G1 (7)

0.1M di-Ammonium sulfate
0.1M TRIS
20% PEG 1500
7.5

G2 (15)

0.2M Sodium acetate
0.1M Sodium citrate
10% PEG 4000
5.5

G3 (23)

0.1M HEPES
15% PEG 4000
7


G4 (31)

20% 2-propanol
0.1M Sodium citrate
20% PEG 4000
5.6

G5 (39)

0.1M Potassium chloride
0.1M HEPES
15% PEG 5000 MME
7

G6 (47)

100 mM TRIS
15% PEG 6000
7.5


G7 (55)

0.1M NaPO4
12% PEG 8000
6.5


G8 (63)

0.1M Magnesium acetate
0.1M MES
10% PEG 10000
6.5

G9 (71)

0.1M Sodium acetate
1.5M di-Ammonium sulfate
5


G10 (79)

0.1M TRIS
3M Na Formate
7.5


G11 (87)

0.1M TRIS
1.6M Lithium sulfate
8


G12 (95)

0.2M Ammonium Acetate
0.1M HEPES
25% 2-propanol
7.5

H1 (8)

0.2M di-Ammonium sulfate
0.1M Sodium acetate
10% PEG 2000 MME
5.5

H2 (16)

0.2M Sodium chloride
0.1M MES
10% PEG 4000
6.5

H3 (24)

0.1M Magnesium chloride
0.1M HEPES
15% PEG 4000
7

H4 (32)

0.2M Sodium chloride
0.1M TRIS
20% PEG 4000
8

H5 (40)

0.2M di-Ammonium sulfate
0.1M TRIS
20% PEG 5000 MME
7.5

H6 (48)

0.1M TRIS
20% PEG 6000
8.5


H7 (56)

0.1M MgAcet
0.1M MOPS 7.5
12% PEG 8000
7.5

H8 (64)

0.1M HEPES
18% PEG 12000
7


H9 (72)

0.1M HEPES
1.5M di-Ammonium sulfate
7


H10 (80)





0.8M K/NaPO4 pH 7.5
H11 (88)





1.4M Na malonate pH 6.0
H12 (96)

0.1M Sodium chloride
5% MPD
0.1M TRIS
15% ethanol