QIAGEN Nucleix crystallisation screen on a 96 well plate (Pipetted in rows)

A1 (1)

0.01 M Magnesium chloride


0.05 M MES pH 5.6
A2 (2)

0.01 M Magnesium acetate


0.05 M MES pH 5.6
A3 (3)

0.1 M Magnesium acetate


0.05 M MES pH 5.6
A4 (4)

0.2 M Potassium chloride
0.01 M Magnesium sulfate

0.05 M MES pH 5.6
A5 (5)

0.2 M Potassium chloride
0.01 M Magnesium chloride

0.05 M MES pH 5.6
A6 (6)

0.1 M Ammonium sulfate
0.01 M Magnesium chloride

0.05 M MES pH 5.6
A7 (7)

0.02 M Magnesium chloride


0.05 M MES pH 6.0
A8 (8)

0.1 M Ammonium acetate
0.005 M Magnesium sulfate

0.05 M MES pH 6.0
A9 (9)

0.1 M Potassium chloride
0.01 M Magnesium chloride

0.05 M MES pH 6.0
A10 (10)

0.005 M Magnesium sulfate


0.05 M MES pH 6.0
A11 (11)

0.01 M Magnesium chloride


0.05 M Sodium cacodylate pH 6.0
A12 (12)

0.01 M Magnesium sulfate


0.05 M Sodium cacodylate pH 6.0
B1 (13)

0.015 M Magnesium acetate


0.05 M Sodium cacodylate pH 6.0
B2 (14)

0.1 M Potassium chloride
0.025 M Magnesium chloride

0.05 M Sodium cacodylate pH 6.0
B3 (15)

0.04 M Magnesium chloride


0.05 M Sodium cacodylate pH 6.0
B4 (16)

0.04 M Magnesium acetate


0.05 M Sodium cacodylate pH 6.0
B5 (17)

0.2 M Potassium chloride
0.01 M Calcium chloride

0.05 M Sodium cacodylate pH 6.0
B6 (18)

0.01 M Magnesium acetate


0.05 M Sodium cacodylate pH 6.5
B7 (19)

0.01 M Magnesium sulfate


0.05 M Sodium cacodylate pH 6.5
B8 (20)

0.1 M Ammonium acetate
0.015 M Magnesium acetate

0.05 M Sodium cacodylate pH 6.5
B9 (21)

0.2 M Potassium chloride
0.005 M Magnesium chloride

0.05 M Sodium cacodylate pH 6.5
B10 (22)

0.08 M Magnesium acetate


0.05 M Sodium cacodylate pH 6.5
B11 (23)

0.2 M Potassium chloride
0.01 M Magnesium chloride

0.05 M Sodium cacodylate pH 6.5
B12 (24)

0.2 M Ammonium acetate
0.01 M Calcium chloride

0.05 M Sodium cacodylate pH 6.5
C1 (25)

0.08 M Magnesium acetate


0.05 M Sodium cacodylate pH 6.5
C2 (26)

0.2 M Potassium chloride
0.1 M Magnesium acetate

0.05 M Sodium cacodylate pH 6.5
C3 (27)

0.2 M Ammonium acetate
0.01 M Magnesium acetate

0.05 M Sodium cacodylate pH 6.5
C4 (28)

0.05 M Magnesium sulfate aq.


0.05 M HEPES Sodium salt pH 7.0
C5 (29)

0.01 M Magnesium chloride


0.05 M HEPES Sodium salt pH 7.0
C6 (30)

0.01 M Magnesium chloride


0.05 M HEPES Sodium salt pH 7.0
C7 (31)

0.005 M Magnesium chloride


0.05 M HEPES Sodium salt pH 7.0
C8 (32)

0.2 M Potassium chloride
0.01 M Magnesium chloride

0.05 M HEPES Sodium salt pH 7.0
C9 (33)

0.2 M Ammonium chloride
0.01 M Magnesium chloride

0.05 M HEPES Sodium salt pH 7.0
C10 (34)

0.1 M Potassium chloride
0.005 M Magnesium sulfate aq.

0.05 M HEPES Sodium salt pH 7.0
C11 (35)

0.1 M Potassium chloride
0.01 M Magnesium chloride

0.05 M HEPES Sodium salt pH 7.0
C12 (36)

0.1 M Potassium chloride
0.01 M Calcium chloride

0.05 M HEPES Sodium salt pH 7.0
D1 (37)

0.2 M Potassium chloride
0.025 M Magnesium sulfate aq.

0.05 M HEPES Sodium salt pH 7.0
D2 (38)

0.2 M Ammonium acetate
0.15 M Magnesium acetate

0.05 M HEPES Sodium salt pH 7.0
D3 (39)

0.1 M Ammonium acetate
0.02 M Magnesium chloride

0.05 M HEPES Sodium salt pH 7.0
D4 (40)

0.01 M Magnesium chloride


0.05 M TRIS.HCl pH 7.5
D5 (41)

0.1 M Potassium chloride
0.015 M Magnesium chloride

0.05 M TRIS.HCl pH 7.5
D6 (42)

0.01 M Magnesium acetate


0.05 M TRIS.HCl pH 7.5
D7 (43)

0.05 M Ammonium acetate
0.01 M Magnesium chloride

0.05 M TRIS.HCl pH 7.5
D8 (44)

0.2 M Potassium chloride
0.05 M Magnesium chloride

0.05 M TRIS.HCl pH 7.5
D9 (45)

0.025 M Magnesium sulfate aq.


0.05 M TRIS.HCl pH 8.5
D10 (46)

0.005 M Magnesium sulfate aq.


0.05 M TRIS.HCl pH 8.5
D11 (47)

0.1 M Potassium chloride
0.01 M Magnesium chloride

0.05 M TRIS.HCl pH 8.5
D12 (48)

0.2 M Ammonium chloride
0.01 M Calcium chloride

0.05 M TRIS.HCl pH 8.5
E1 (49)

2.5 mM Spermine


0.05 M HEPES pH 7.5
E2 (50)

2.25 mM Spermine
18 mM Magnesium chloride
9 %(v/v) Isopropanol
0.05 M Sodium cacodylate pH 6.0
E3 (51)

0.9 mM Spermine
18 mM Magnesium chloride
9 %(v/v) Isopropanol
0.05 M Sodium cacodylate pH 6.5
E4 (52)

2.25 mM Spermine
18 mM Magnesium chloride

0.05 M Sodium cacodylate pH 6.5
E5 (53)

2.25 mM Spermine
18 mM Magnesium chloride
4.5 %(v/v) MPD
0.05 M Sodium cacodylate pH 7.0
E6 (54)

2.25 mM Spermine
36 mM Magnesium chloride

0.05 M Sodium cacodylate pH 6.5
E7 (55)

2.0 mM Cobalt chloride
10 mM Magnesium chloride

0.05 M Sodium succinate pH 5.5
E8 (56)

1.0 mM Spermine
20 mM Magnesium chloride

0.05 M Sodium cacodylate pH 6.0
E9 (57)

1.0 mM Spermine
20 mM Magnesium chloride
15 %(v/v) Ethanol
0.05 M Sodium cacodylate pH 7.0
E10 (58)

1.0 mM Spermidine
5 mM Magnesium chloride

0.05 M Sodium cacodylate pH 7.0
E11 (59)

2.5 mM Spermine
30 mM Magnesium chloride

0.05 M Sodium cacodylate pH 7.0
E12 (60)

2.0 mM Cobalt chloride
100 mM Magnesium chloride

0.05 M Sodium cacodylate pH 6.5
F1 (61)

1.0 mM Cobalt chloride
10 mM Magnesium chloride

0.05 M TRIS pH 8.0
F2 (62)

1.0 mM Spermine
20 mM Magnesium chloride

0.05 M HEPES pH 7.5
F3 (63)

2.5 mM Spermine
20 mM Magnesium chloride

0.05 M Sodium cacodylate pH 6.0
F4 (64)

2.5 mM Spermine
10 mM Magnesium chloride
10 %(v/v) Isopropanol
0.05 M Sodium cacodylate pH 6.0
F5 (65)

2.25 mM Spermine
9 mM Magnesium chloride
5 % (v/v) PEG 400
0.05 M Sodium cacodylate pH 7.0
F6 (66)

2.5 mM Spermine
10 mM Magnesium chloride
10 %(v/v) Isopropanol
0.05 M Sodium cacodylate pH 6.5
F7 (67)

1.0 mM Spermine
20 mM Magnesium chloride
10 %(v/v) 1,5-Hexandiol
0.05 M Sodium cacodylate pH 6.0
F8 (68)

1.0 mM Spermidine
15 mM Magnesium chloride

0.05 M HEPES pH 7.5
F9 (69)

3.0 mM Spermine
15 mM Magnesium chloride

0.05 M Sodium cacodylate pH 6.0
F10 (70)

18 mM Calcium chloride
2.5 mM Spermine

0.05 M Sodium cacodylate pH 6.5
F11 (71)

1.0 mM Cobalt chloride
2.0 mM Spermine

0.05 M Sodium cacodylate pH 6.5
F12 (72)

2.5 mM Cobalt chloride
5 mM Magnesium chloride

0.05 M Sodium cacodylate pH 6.5
G1 (73)

1.0 mM Spermine
30 mM Magnesium chloride

0.05 M Sodium cacodylate pH 6.5
G2 (74)

200 mM Calcium acetate
5 %(v/v) Isopropanol

0.05 M Sodium cacodylate pH 6.0
G3 (75)

1.0 mM Cobalt chloride
100 mM Magnesium chloride

0.05 M Sodium cacodylate pH 6.5
G4 (76)

2.5 mM Spermidine
10 mM Magnesium chloride

0.05 M Sodium cacodylate pH 6.0
G5 (77)

200 mM tri-Na citrate
10 mM Magnesium chloride

0.05 M Sodium cacodylate pH 6.5
G6 (78)

10.0 mM Spermine
15 mM Magnesium chloride

0.05 M Sodium cacodylate pH 6.5
G7 (79)

1.0 mM Spermine
20 mM Magnesium chloride

0.05 M Sodium cacodylate pH 6.5
G8 (80)

1.5 mM Spermine
10 mM Magnesium chloride

0.05 M Sodium cacodylate pH 6.5
G9 (81)

1.0 mM Spermine
15 mM Magnesium chloride

0.05 M HEPES pH 7.5
G10 (82)

200 mM Calcium acetate
2.5 M Sodium chloride

0.05 M Sodium cacodylate pH 6.0
G11 (83)

1.0 mM Cobalt chloride
200 mM Calcium acetate

0.05 M Sodium cacodylate pH 6.0
G12 (84)

5.0 mM Spermidine
15 mM Magnesium chloride
2.0 M Sodium chloride
0.05 M Sodium cacodylate pH 6.5
H1 (85)

100 mM Sodium chloride
200 mM Magnesium chloride

0.05 M Sodium cacodylate pH 6.5
H2 (86)

50 mM Magnesium chloride


0.05 M TRIS pH 7.5
H3 (87)

200 mM Magnesium chloride


0.05 M TRIS pH 7.5
H4 (88)

200 mM Magnesium chloride


0.05 M Sodium cacodylate pH 6.0
H5 (89)

200 mM Magnesium chloride


0.05 M TRIS pH 8.0
H6 (90)

5.0 mM Spermidine
15 mM Magnesium chloride

0.05 M Sodium cacodylate pH 6.0
H7 (91)

0.5 mM Spermine
20 mM Magnesium acetate
25 %(v/v) MPD
0.05 M Sodium cacodylate pH 6.0
H8 (92)

0.5 mM Spermine
20 mM Magnesium chloride

0.05 M Sodium succinate pH 5.5
H9 (93)

5 mM Cobalt chloride
2.5 M Potassium chloride

0.05 M Sodium cacodylate pH 6.5
H10 (94)

2.0 mM Cobalt chloride
50 mM Magnesium chloride

0.05 M Sodium cacodylate pH 6.5
H11 (95)

2.0 mM Cobalt chloride
1.0 mM Spermine
2.0 M Lithium chloride
0.05 M Sodium cacodylate pH 6.5
H12 (96)

50 mM Spermine


0.05 M Sodium cacodylate pH 6.5