QIAGEN Cryos crystallisation screen on a 96 well plate (Pipetted in columns)

A1 (1)

0.0085 M Cobalt chloride
0.85 M 1,6-Hexanediol
15 %(v/v) Glycerol

0.085 M Sodium acetate pH 4.6
A2 (9)

0.14 M tri-Sodium citrate
21 %(v/v) Isopropanol
30 %(v/v) Glycerol

0.07 M Sodium cacodylate pH 6.5
A3 (17)

0.18 M Ammonium acetate
27 %(v/v) MPD
10 %(v/v) Glycerol

0.09 M tri-Sodium citrate pH 5.6
A4 (25)

0.26 M Ammonium phosphate
35 %(v/v) Glycerol



A5 (33)

0.0085 M Cobalt chloride
1.53 M Ammonium sulfate
15 %(v/v) Glycerol

0.085 M MES pH 6.5
A6 (41)

1.7 M Sodium chloride
15 %(v/v) Glycerol


0.085 M Sodium acetate pH 4.6
A7 (49)

1.7 %(v/v) Dioxane, 8.5 %(w/v) PEG 20000
15 %(v/v) Glycerol


0.085 M BICINE pH 9
A8 (57)

0.425 M Ammonium sulfate
0.85 M Lithium sulfate
15 %(v/v) Glycerol

0.085 M tri-Sodium citrate pH 5.6
A9 (65)

0.4 M Lithium sulfate
12 %(w/v) PEG 8000
20 %(v/v) Glycerol


A10 (73)

1.7 %(v/v) PEG 400, 1.7 M Ammonium sulfate
15 %(v/v) Glycerol


0.085 M HEPES sodium salt pH 7.5
A11 (81)

24 %(w/v) PEG 1500
20 %(v/v) Glycerol



A12 (89)

0.17 M Lithium sulfate
25.5 %(w/v) PEG 4000
15 %(v/v) Glycerol

0.085 M TRIS.HCl pH 8.5
B1 (2)

2.125 M 1,6-Hexanediol
15 %(v/v) Glycerol


0.085 M tri-Sodium citrate pH 5.6
B2 (10)

0.18 M Magnesium chloride
27 %(v/v) Isopropanol
10 %(v/v) Glycerol

0.09 M HEPES sodium salt pH 7.5
B3 (18)

0.18 M Magnesium acetate
27 %(v/v) MPD
10 %(v/v) Glycerol

0.09 M Sodium cacodylate pH 6.5
B4 (26)

0.7 M Ammonium phosphate
30 %(v/v) Glycerol


0.07 M tri-Sodium citrate pH 5.6
B5 (34)

0.17 M K/Na tartrate
1.7 M Ammonium sulfate
15 %(v/v) Glycerol

0.085 M tri-Sodium citrate pH 5.6
B6 (42)

0.085 M Sodium phosphate
0.085 M Potassium phosphate
1.7 M Sodium chloride
15 %(v/v) Glycerol
0.085 M MES pH 6.5
B7 (50)

8.5 %(v/v) Dioxane, 1.36 M Ammonium sulfate
15 %(v/v) Glycerol


0.085 M MES pH 6.5
B8 (58)

0.0085 M Nickel chloride
0.85 M Lithium sulfate
15 %(v/v) Glycerol

0.085 M TRIS pH 8.5
B9 (66)

0.16 M Zinc acetate
14.4 %(w/v) PEG 8000
20 %(v/v) Glycerol

0.08 M Sodium cacodylate pH 6.5
B10 (74)

0.19 M Calcium chloride
26.6 %(v/v) PEG 400
5 %(v/v) Glycerol

0.095 M HEPES sodium salt pH 7.5
B11 (82)

0.0085 M Nickel chloride
17 %(w/v) PEG 2000 MME
15 %(v/v) Glycerol

0.085 M TRIS pH 8.5
B12 (90)

0.17 M Sodium acetate
25.5 %(w/v) PEG 4000
15 %(v/v) Glycerol

0.085 M TRIS.HCl pH 8.5
C1 (3)

0.17 M Magnesium chloride
2.89 M 1,6-Hexanediol
15 %(v/v) Glycerol

0.085 M TRIS pH 8.5
C2 (11)

0.16 M Ammonium acetate
24 %(v/v) Isopropanol
20 %(v/v) Glycerol

0.08 M TRIS.HCl pH 8.5
C3 (19)

0.18 M tri-Sodium citrate
27 %(v/v) MPD
10 %(v/v) Glycerol

0.09 M HEPES sodium salt pH 7.5
C4 (27)

1.6 M Ammonium phosphate
20 %(v/v) Glycerol


0.08 M TRIS.HCl pH 8.5
C5 (35)

15 %(v/v) Glycerol



0.85 M Imidazole pH 7.0
C6 (43)

3.655 M Sodium chloride
15 %(v/v) Glycerol


0.085 M HEPES pH 7.5
C7 (51)

29.75 %(v/v) Dioxane
15 %(v/v) Glycerol



C8 (59)

1.125 M Lithium sulfate
25 %(v/v) Glycerol


0.075 M HEPES sodium salt pH 7.5
C9 (67)

0.16 M Calcium acetate
14.4 %(w/v) PEG 8000
20 %(v/v) Glycerol

0.08 M Sodium cacodylate pH 6.5
C10 (75)

0.085 M Cadmium chloride
25.5 %(v/v) PEG 400
15 %(v/v) Glycerol

0.085 M Sodium acetate pH 4.6
C11 (83)

0.17 M Ammonium sulfate
25.5 %(w/v) PEG 2000 MME
15 %(v/v) Glycerol

0.085 M Sodium acetate pH 4.6
C12 (91)

0.17 M Ammonium sulfate
25.5 %(w/v) PEG 4000
15 %(v/v) Glycerol


D1 (4)

4.25 %(v/v) Isopropanol, 1.7 M Ammonium sulfate
15 %(v/v) Glycerol



D2 (12)

8.5 %(v/v) Ethanol, 1.275 M Sodium chloride
15 %(v/v) Glycerol



D3 (20)

0.425 M Ammonium sulfate
25.5 %(v/v) MPD
15 %(v/v) Glycerol

0.085 M HEPES pH 7.5
D4 (28)

1.7 M Ammonium formate
15 %(v/v) Glycerol


0.085 M HEPES pH 7.5
D5 (36)

0.26 M K/Na tartrate
35 %(v/v) Glycerol



D6 (44)

1.26 M tri-Sodium citrate
10 %(v/v) Glycerol


0.09 M HEPES sodium salt pH 7.5
D7 (52)

0.425 M Sodium chloride
1.7 %(v/v) Ethylene imine polymer
15 %(v/v) Glycerol

0.085 M tri-Sodium citrate pH 5.6
D8 (60)

1.7 M Magnesium chloride
15 %(v/v) Glycerol


0.085 M BICINE pH 9.0
D9 (68)

0.16 M Magnesium acetate
16 %(w/v) PEG 8000
20 %(v/v) Glycerol

0.08 M Sodium cacodylate pH 6.5
D10 (76)

0.18 M Magnesium chloride
27 %(v/v) PEG 400
10 %(v/v) Glycerol

0.09 M HEPES sodium salt pH 7.5
D11 (84)

5.6 %(w/v) PEG 4000
30 %(v/v) Glycerol


0.07 M Sodium acetate pH 4.6
D12 (92)

0.17 M Ammonium sulfate
25.5 %(w/v) PEG 5000 MME
15 %(v/v) Glycerol

0.085 M MES pH 6.5
E1 (5)

8.5 %(v/v) Isopropanol, 17 %(w/v) PEG 4000
15 %(v/v) Glycerol


0.085 M HEPES sodium salt pH 7.5
E2 (13)

17 %(v/v) Ethanol
15 %(v/v) Glycerol


0.085 M TRIS pH 8.5
E3 (21)

0.17 M Ammonium phosphate
42.5 %(v/v) MPD
15 %(v/v) Glycerol

0.085 M TRIS pH 8.5
E4 (29)

1.6 M Ammonium sulfate
20 %(v/v) Glycerol


0.08 M Sodium acetate pH 4.6
E5 (37)

0.52 M K/Na tartrate
35 %(v/v) Glycerol


0.065 M HEPES sodium salt pH 7.5
E6 (45)

15 %(v/v) Glycerol



1.36 M tri-Sodium citrate pH 6.5
E7 (53)

10.2 %(v/v) Glycerol,
1.275 M Ammonium sulfate
15 %(v/v) Glycerol

0.085 M TRIS pH 8.5
E8 (61)

0.17 M Magnesium formate
15 %(v/v) Glycerol



E9 (69)

0.04 M Potassium phosphate
16 %(w/v) PEG 8000
20 %(v/v) Glycerol


E10 (77)

0.18 M tri-Sodium citrate
27 %(v/v) PEG 400
10 %(v/v) Glycerol

0.09 M TRIS.HCl pH 8.5
E11 (85)

0.16 M Ammonium sulfate
20 %(w/v) PEG 4000
20 %(v/v) Glycerol

0.08 M Sodium acetate pH 4.6
E12 (93)

8.5 %(w/v) PEG 6000, 4.25 %(v/v) MPD
15 %(v/v) Glycerol


0.085 M HEPES pH 7.5
F1 (6)

0.14 M Calcium chloride
14 %(v/v) Isopropanol
30 %(v/v) Glycerol

0.07 M Sodium acetate pH 4.6
F2 (14)

21.25 %(v/v) Ethylene glycol
15 %(v/v) Glycerol



F3 (22)

59.5 %(v/v) MPD
15 %(v/v) Glycerol


0.085 M HEPES pH 7.5
F4 (30)

1.5 M Ammonium sulfate
25 %(v/v) Glycerol


0.075 M TRIS.HCl pH 8.5
F5 (38)

0.7 M Sodium acetate
30 %(v/v) Glycerol


0.07 M Imidazole pH 6.5
F6 (46)

0.6 M Sodium phosphate,
0.6 M Potassium phosphate
25 %(v/v) Glycerol

0.075 M HEPES sodium salt pH 7.5
F7 (54)

0.425 M Sodium chloride
0.085 Magnesium chloride
0.0085 M CTAB
15 %(v/v) Glycerol

F8 (62)

1.36 M Magnesium sulfate
15 %(v/v) Glycerol


0.085 M MES pH 6.5
F9 (70)

0.17 M Ammonium sulfate
25.5 %(w/v) PEG 8000
15 %(v/v) Glycerol

0.085 M Sodium cacodylate pH 6.5
F10 (78)

0.085 M Sodium chloride
17 %(w/v) PEG 550 MME
15 %(v/v) Glycerol

0.085 M BICINE pH 9.0
F11 (86)

0.17 M Ammonium acetate
25.5 %(w/v) PEG 4000
15 %(v/v) Glycerol

0.085 M Sodium acetate pH 4.6
F12 (94)

8 %(w/v) PEG 6000, 1.6 M Sodium chloride
20 %(v/v) Glycerol



G1 (7)

19 %(v/v) Isopropanol, 19 %(w/v) PEG 4000
5 %(v/v) Glycerol


0.095 M tri-Sodium citrate pH 5.6
G2 (15)

0.018 M Calcium chloride
27 %(v/v) MPD
10 %(v/v) Glycerol

0.09 M Sodium acetate pH 4.6
G3 (23)

21.25 %(v/v) tert-Butanol
15 %(v/v) Glycerol


0.085 M TRIS pH 8.5
G4 (31)

1.5 M Ammonium sulfate
25 %(v/v) Glycerol



G5 (39)

0.0425 M Cadmium sulfate
0.85 M Sodium acetate
15 %(v/v) Glycerol

0.085 M HEPES pH 7.5
G6 (47)

1.4 M Sodium formate
30 %(v/v) Glycerol


0.07 M Sodium acetate pH 4.6
G7 (55)

0.0085 M Ferric chloride
8.5 %(v/v) Jeffamine M-600
15 %(v/v) Glycerol

0.085 M tri-Sodium citrate pH 5.6
G8 (63)

5.2 %(w/v) PEG 8000
35 %(v/v) Glycerol


0.065 M TRIS.HCl pH 8.5
G9 (71)

0.17 M Sodium acetate
25.5 %(w/v) PEG 8000
15 %(v/v) Glycerol

0.085 M Sodium cacodylate pH 6.5
G10 (79)

0.0085 M Zinc sulfate
21.25 %(w/v) PEG 550 MME
15 %(v/v) Glycerol

0.085 M MES pH 6.5
G11 (87)

0.17 M Ammonium acetate
25.5 %(w/v) PEG 4000
15 %(v/v) Glycerol

0.085 M tri-Sodium citrate pH 5.6
G12 (95)

17 %(w/v) PEG 10000, 6.8 % Ethylene glycol
15 %(v/v) Glycerol


0.085 M HEPES pH 7.5
H1 (8)

0.14 M tri-Sodium citrate
14 %(v/v) Isopropanol
30 %(v/v) Glycerol

0.07 M HEPES sodium salt pH 7.5
H2 (16)

0.17 M Sodium chloride
25.5 %(v/v) MPD
15 %(v/v) Glycerol

0.085 M Sodium acetate pH 4.6
H3 (24)

29.75 %(v/v) tert-Butanol
15 %(v/v) Glycerol


0.085 M tri-Sodium citrate pH 5.6
H4 (32)

0.085 M Sodium chloride
1.36 M Ammonium sulfate
15 %(v/v) Glycerol

0.085 M HEPES pH 7.5
H5 (40)

0.98 M Sodium acetate
30 %(v/v) Glycerol


0.07 M Sodium cacodylate pH 6.5
H6 (48)

3.6 M Sodium formate
10 %(v/v) Glycerol



H7 (56)

17 %(v/v) Jeffamine M-600
15 %(v/v) Glycerol


0.085 M HEPES pH 7.5
H8 (64)

8.5 %(w/v) PEG 8000
15 %(v/v) Glycerol


0.085 M HEPES pH 7.5
H9 (72)

0.17 M Ammonium sulfate
25.5 %(w/v) PEG 8000
15 %(v/v) Glycerol


H10 (80)

8.5 %(w/v) PEG 1000, 8.5 %(w/v) PEG 8000
15 %(v/v) Glycerol



H11 (88)

0.16 M Magnesium chloride
24 %(w/v) PEG 4000
20 %(v/v) Glycerol

0.08 M TRIS.HCl pH 8.5
H12 (96)

10.2 %(w/v) PEG 20000
15 %(v/v) Glycerol


0.085 M MES pH 6.5