QIAGEN ClassicsII crystallisation screen on a 96 well plate (Pipetted in columns)

A1 (1)

2 M Ammonium sulfate



0.1 M Citric acid pH 3.5
A2 (9)

3 M Sodium chloride



0.1 M BIS-TRIS pH 5.5
A3 (17)

1.26 M Sodium phosphate
0.14 M Potassium phosphate



A4 (25)





3.5 M Sodium formate pH 7.0
A5 (33)





0.5 %(v/v) Jeffamine ED-2001 pH 7.0
A6 (41)

25 %(w/v) PEG 3350



0.1 M Sodium acetate pH 4.5
A7 (49)

0.2 M Calcium chloride
45 %(v/v) MPD


0.1 M BIS-TRIS pH 6.5
A8 (57)

0.05 M Ammonium sulfate
30 %(v/v) Pentaerythritol ethoxylate


0.05 M BIS-TRIS pH 6.5
A9 (65)

0.1 M Ammonium acetate
17 %(w/v) PEG 10,000


0.1 M BIS-TRIS pH 5.5
A10 (73)

0.2 M Sodium chloride
25 %(w/v) PEG 3350


0.1 M TRIS pH 8.5
A11 (81)

0.2 M Ammonium acetate
25 %(w/v) PEG 3350


0.1 M TRIS pH 8.5
A12 (89)

15 %(w/v) PEG 3350



0.1 M Succinic acid pH 7.0
B1 (2)

2 M Ammonium sulfate



0.1 M Sodium acetate pH 4.5
B2 (10)

3 M Sodium chloride



0.1 M BIS-TRIS pH 6.5
B3 (18)

0.49 M Sodium phosphate
0.91 M Potassium phosphate



B4 (26)





1.1 M Ammonium tartrate pH 7.0
B5 (34)

1 %(w/v) PEG 2000 MME



0.1 M HEPES pH 7.0
B6 (42)

25 %(w/v) PEG 3350



0.1 M BIS-TRIS pH 5.5
B7 (50)

0.2 M Ammonium acetate
45 %(v/v) MPD


0.1 M BIS-TRIS pH 5.5
B8 (58)

45 %(v/v) PEG P 400



0.1 M BIS-TRIS pH 6.5
B9 (66)

0.2 M Ammonium sulfate
25 %(w/v) PEG 3350


0.1 M BIS-TRIS pH 5.5
B10 (74)

0.2 M Lithium sulfate
25 %(w/v) PEG 3350


0.1 M BIS-TRIS pH 5.5
B11 (82)

0.2 M Magnesium chloride
25 %(w/v) PEG 3350


0.1 M BIS-TRIS pH 5.5
B12 (90)

0.2 M Sodium formate
20 %(w/v) PEG 3350



C1 (3)

2 M Ammonium sulfate



0.1 M BIS-TRIS pH 5.5
C2 (11)

3 M Sodium chloride



0.1 M HEPES pH 7.5
C3 (19)

0.056 M Sodium phosphate
1.344 M Potassium phosphate



C4 (27)





2.4 M Sodium malonate pH 7.0
C5 (35)

1 M Ammonium sulfate
0.5 %(w/v) PEG 8000


0.1 M HEPES pH 7.0
C6 (43)

25 %(w/v) PEG 3350



0.1 M BIS-TRIS pH 6.5
C7 (51)

0.2 M Ammonium acetate
45 %(v/v) MPD


0.1 M BIS-TRIS pH 6.5
C8 (59)

0.02 M Magnesium chloride
22 %(w/v) Sodium Polyacrylate 5100


0.1 M HEPES pH 7.5
C9 (67)

0.2 M Ammonium sulfate
25 %(w/v) PEG 3350


0.1 M BIS-TRIS pH 6.5
C10 (75)

0.2 M Lithium sulfate
25 %(w/v) PEG 3350


0.1 M BIS-TRIS pH 6.5
C11 (83)

0.2 M Magnesium chloride
25 %(w/v) PEG 3350


0.1 M BIS-TRIS pH 6.5
C12 (91)

20 %(w/v) PEG 3350



0.15 M DL-Malic acid pH 7.0
D1 (4)

2 M Ammonium sulfate



0.1 M BIS-TRIS pH 6.5
D2 (12)

3 M Sodium chloride



0.1 M TRIS pH 8.5
D3 (20)

1.4 M Sodium citrate



0.1 M HEPES pH 7.5
D4 (28)





0.56 M Sodium citrate pH 7.0
D5 (36)

2 %(w/v) PEG 3350



0.1 M HEPES pH 7.0
D6 (44)

25 %(w/v) PEG 3350



0.1 M HEPES pH 7.5
D7 (52)

0.2 M Ammonium acetate
45 %(v/v) MPD


0.1 M HEPES pH 7.5
D8 (60)

0.01 M Cobalt chloride
20 %(w/v) PVP K15


0.1 M TRIS pH 8.5
D9 (68)

0.2 M Ammonium sulfate
25 %(w/v) PEG 3350


0.1 M HEPES pH 7.5
D10 (76)

0.2 M Lithium sulfate
25 %(w/v) PEG 3350


0.1 M HEPES pH 7.5
D11 (84)

0.2 M Magnesium chloride
25 %(w/v) PEG 3350


0.1 M HEPES pH 7.5
D12 (92)

0.1 M Magnesium formate
15 %(w/v) PEG 3350



E1 (5)

2 M Ammonium sulfate



0.1 M HEPES pH 7.5
E2 (13)

0.3 M Magnesium formate



0.1 M BIS-TRIS pH 5.5
E3 (21)





1.8 M Ammonium citrate pH 7.0
E4 (29)





0.96 M Sodium citrate pH 7.0
E5 (37)

25 %(w/v) PEG 1500




E6 (45)

25 %(w/v) PEG 3350



0.1 M TRIS pH 8.5
E7 (53)

0.2 M Ammonium acetate
45 %(v/v) MPD


0.1 M TRIS pH 8.5
E8 (61)

0.2 M L-Proline
10 %(w/v) PEG 3350


0.1 M HEPES pH 7.5
E9 (69)

0.2 M Ammonium sulfate
25 %(w/v) PEG 3350


0.1 M TRIS pH 8.5
E10 (77)

0.2 M Lithium sulfate
25 %(w/v) PEG 3350


0.1 M TRIS pH 8.5
E11 (85)

0.2 M Magnesium chloride
25 %(w/v) PEG 3350


0.1 M TRIS pH 8.5
E12 (93)

0.05 M Zinc acetate
20 %(w/v) PEG 3350



F1 (6)

2 M Ammonium sulfate



0.1 M TRIS pH 8.5
F2 (14)

0.5 M Magnesium formate



0.1 M BIS-TRIS pH 6.5
F3 (22)





0.8 M Succinic acid pH 7.0
F4 (30)

0.1 M Sodium chloride
1.5 M Ammonium sulfate


0.1 M BIS-TRIS pH 6.5
F5 (38)





30 %(v/v) Jeffamine M-600 pH 7.0
F6 (46)

20 %(w/v) PEG 5000 MME



0.1 M BIS-TRIS pH 6.5
F7 (54)

0.05 M Calcium chloride
30 %(v/v) PEG 550 MME


0.1 M BIS-TRIS pH 6.5
F8 (62)

0.2 M TMAO
20 %(w/v) PEG 2000 MME


0.1 M TRIS pH 8.5
F9 (70)

0.2 M Sodium chloride
25 %(w/v) PEG 3350


0.1 M BIS-TRIS pH 5.5
F10 (78)

0.2 M Ammonium acetate
25 %(w/v) PEG 3350


0.1 M BIS-TRIS pH 5.5
F11 (86)

0.2 M Potassium Sodium tartrate
20 %(w/v) PEG 3350



F12 (94)

0.2 M Sodium citrate
20 %(w/v) PEG 3350



G1 (7)

3 M Sodium chloride



0.1 M Citric acid pH 3.5
G2 (15)

0.5 M Magnesium formate



0.1 M HEPES pH 7.5
G3 (23)





2.1 M DL-Malic acid pH 7.0
G4 (31)

0.8 M Sodium/Potassium tartrate
0.5 %(w/v) PEG 5000 MME


0.1 M TRIS pH 8.5
G5 (39)





30 %(v/v) Jeffamine ED-2001 pH 7.0
G6 (47)

28 %(w/v) PEG 2000 MME



0.1 M BIS-TRIS pH 6.5
G7 (55)

0.05 M Magnesium chloride
30 %(v/v) PEG 550 MME


0.1 M HEPES pH 7.5
G8 (63)

10 %(w/v) PEG 5000 MME



0.1 M HEPES pH 7.0
G9 (71)

0.2 M Sodium chloride
25 %(w/v) PEG 3350


0.1 M BIS-TRIS pH 6.5
G10 (79)

0.2 M Ammonium acetate
25 %(w/v) PEG 3350


0.1 M BIS-TRIS pH 6.5
G11 (87)

20 %(w/v) PEG 3350



0.2 M Sodium malonate pH 7.0
G12 (95)

0.1 M Potassium thiocyanate
30 %(w/v) PEG 2000 MME



H1 (8)

3 M Sodium chloride



0.1 M Sodium acetate pH 4.5
H2 (16)

0.3 M Magnesium formate



0.1 M TRIS pH 8.5
H3 (24)





2.8 M Sodium acetate pH 7.0
H4 (32)

1 M Ammonium sulfate
1 %(w/v) PEG 3350


0.1 M BIS-TRIS pH 5.5
H5 (40)

25 %(w/v) PEG 3350



0.1 M Citric acid pH 3.5
H6 (48)

0.2 M Calcium chloride
45 %(v/v) MPD


0.1 M BIS-TRIS pH 5.5
H7 (56)

0.2 M Potassium chloride
35 %(v/v) Pentaerythritol propoxylate


0.05 M HEPES pH 7.5
H8 (64)

0.005 M Cobalt chloride
0.005 M Nickel chloride
0.005 M Cadmium chloride
0.005 M Magnesium chloride, 12 %(w/v) PEG 3350
0.1 M HEPES pH 7.5
H9 (72)

0.2 M Sodium chloride
25 %(w/v) PEG 3350


0.1 M HEPES pH 7.5
H10 (80)

0.2 M Ammonium acetate
25 %(w/v) PEG 3350


0.1 M HEPES pH 7.5
H11 (88)

20 %(w/v) PEG 3350



0.2 M Ammonium citrate pH 7.0
H12 (96)

0.15 M Potassium bromide
30 %(w/v) PEG 2000 MME